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A person obstacle during the cross-study comparability of qPCR-dependent quantitative microbiome profiling is definitely the reliance on an external qPCR conventional from a reference organism required to construct a standard curve. In idea, any standard taxon present inside a microbial Local community of curiosity can be utilized because the reference organism for standard curve building.
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Importantly, qPCR-centered quantitative microbiome profiling enjoys the following conceptual and useful Gains about other approaches:
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